Vitamin B6 in Plasma and Cerebrospinal Fluid of Children
نویسندگان
چکیده
BACKGROUND Over the past years, the essential role of vitamin B6 in brain development and functioning has been recognized and genetic metabolic disorders resulting in functional vitamin B6 deficiency have been identified. However, data on B6 vitamers in children are scarce. MATERIALS AND METHODS B6 vitamer concentrations in simultaneously sampled plasma and cerebrospinal fluid (CSF) of 70 children with intellectual disability were determined by ultra performance liquid chromatography-tandem mass spectrometry. For ethical reasons, CSF samples could not be obtained from healthy children. The influence of sex, age, epilepsy and treatment with anti-epileptic drugs, were investigated. RESULTS The B6 vitamer composition of plasma (pyridoxal phosphate (PLP) > pyridoxic acid > pyridoxal (PL)) differed from that of CSF (PL > PLP > pyridoxic acid > pyridoxamine). Strong correlations were found for B6 vitamers in and between plasma and CSF. Treatment with anti-epileptic drugs resulted in decreased concentrations of PL and PLP in CSF. CONCLUSION We provide concentrations of all B6 vitamers in plasma and CSF of children with intellectual disability (±epilepsy), which can be used in the investigation of known and novel disorders associated with vitamin B6 metabolism as well as in monitoring of the biochemical effects of treatment with vitamin B6.
منابع مشابه
The effect of Vitamin B6 on chemotherapy induced nausea and vomiting in pediatric cancer
Background: Nausea and vomiting are the common side-effects of chemotherapy in children with malignancy. In this study, the effectiveness of vitamin B6 in reducing the chemo-induced nausea and vomiting (CINV) in children was tested. Material and methods: A triple-blind clinical trials was performed on 100 children with malignancy referring to the pediatric clinic of Amir Kabir Hospital, Arak, ...
متن کاملMorphine in Plasma and Cerebrospinal Fluid of Patients Addicted to Opiates Undergoing Surgery: High-performance Liquid Chromatography Method
Background: The prevalence of opium addiction among Iranians is considerable. Since endogenous opioid systems may be altered as a consequence of addiction, it is very important to determine the plasma and cerebrospinal fluid (CSF) levels of morphine in Iranian patients addicted to opiates who will undergo surgery.Methods: We obtained CSF and plasma samples from 50 volunteers with an established...
متن کاملFrequency of Neonatal and children bacterial meningitis and determining their antibiotic sensitivity patterns in Hospitals of Iran
Meningitis is one of the most important causes of infant mortality and its early and proper diagnosis is of great importance, but there are currently no laboratory facilities optimized and fast in detection of meningitis pathogens. The current standard of bacterial meningitis diagnosis is microscopic examination and cerebrospinal fluid (CSF) culture. This research aimed at isolating the most co...
متن کاملThe Comparison of Leukemia Inhibitory Factor (LIF) Concentration in the Serum and Cerebrospinal Fluid of Children with Bacterial Meningitis
Abstract Background and objectives: Meningitis is one of the most common infectious of the central nervous system (CNS), defined as an inflammation of the meninges. LIF is a potent pro-inflammatory factor. Cerebrospinal fluid (CSF) contains the growth factors and cytokines whose concentrations have been changed in most neurological diseases. The aim of this study was to determine the LIF conce...
متن کاملCerebrospinal fluid nerve growth factor and total protein concentration in the children with meningitis
Meningitis is one of the most common infectious cerebral nervous system (CNS), defined as an inflammation of the meninges. It is clinically categorized into a chronic and acute based on the acuity of symptoms. Vomiting, bulging fontanel and fever are the main symptoms in the patients with meningitis. Bacterial meningitis is a severe, potentially life-threatening infection that is associated wit...
متن کامل